apc nt5e cd73 monoclonal antibody ad2  (Thermo Fisher)

Journal: bioRxiv

Article Title: The AP-1 factors FOSL1 and FOSL2 co-regulate human Th17 responses

doi: 10.1101/2021.04.26.441472

Figure Lengend Snippet: ( A and B ) Heatmap shows scaled expression values for the differentially expressed (DE) genes (FDR ≤ 0.1) in FOSL DOE (panel A) and FOSL DKD (panel B) Th17 cells. DOE targets with |fold change| ≥ 2 and DKD targets with |fold change| ≥ 1.8 are selectively shown. For the DKD heatmap, only the genes with statistically significant expression values at both time points are depicted. Targets associated with Th17-function are highlighted. ( C ) Venn diagram shows the overlap for the DKD gene-targets identified at 24h and 72h of Th17 polarization (FDR ≤ 0.1, |fold change| ≥ 1.8). ( D ) CCR6 expression was analyzed by flow-cytometry in FOSL OE/DOE [left] or KD/DKD [right] Th17 cells, at 72h of polarization. Median fluorescence intensity (MFI) values were normalized to the respective controls (SCR or Empty GFP) and plotted. ( E ) Figure shows flow-cytometry analysis of NT5E [left] and CD70 [right] protein expression in FOSL KD/DKD Th17 cells, at 72h of polarization. Bar plots show MFI values that are normalized to control. For panels D and E, data shows mean ± standard error of the mean (SEM) for three or four biological replicates, as indicated. Statistical significance was calculated using two-tailed Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001).

Article Snippet: The following antibodies were used for flow cytometry: anti-CCR6 PE (BD Cat no. 559562); anti-FOSL1 (Santacruz Biotechnology, Cat no. sc-28310); anti-FOSL2 (Cell Signaling Tech., Cat no.19967); APC-NT5E (CD73) monoclonal antibody (AD2) (Thermo Fischer, Cat no.17-0739-42), PE anti-human CD70 antibody (Biolegend, Cat no. 355103).

Techniques: Expressing, Flow Cytometry, Fluorescence, Two Tailed Test

Journal: bioRxiv

Article Title: The AP-1 factors FOSL1 and FOSL2 co-regulate human Th17 responses

doi: 10.1101/2021.04.26.441472

Figure Lengend Snippet: ( A-D ) Western blots show protein-level expression of STAT4, NT5E, APOD and JUNB in FOSL KD and DKD Th17 cells at 72h of polarization. Immunoblots (below panel) were quantified using ImageJ and the corresponding FOSL intensity values were plotted into graphs (above panel). Actin served as loading control and was used for normalization. The JUNB and STAT4 blots for replicate 4 share the same loading control. Data shows mean ± SEM for three (NT5E, APOD) or four biological replicates (JUNB, STAT4). Statistical significance was calculated using two-tailed Student’s t test (*p < 0.05; **p < 0.01).

Article Snippet: The following antibodies were used for flow cytometry: anti-CCR6 PE (BD Cat no. 559562); anti-FOSL1 (Santacruz Biotechnology, Cat no. sc-28310); anti-FOSL2 (Cell Signaling Tech., Cat no.19967); APC-NT5E (CD73) monoclonal antibody (AD2) (Thermo Fischer, Cat no.17-0739-42), PE anti-human CD70 antibody (Biolegend, Cat no. 355103).

Techniques: Western Blot, Expressing, Two Tailed Test

Journal: Biomedicines

Article Title: The Effects of Immunosuppressive Drugs on the Characteristics and Functional Properties of Bone Marrow-Derived Stem Cells Isolated from Patients with Diabetes Mellitus and Peripheral Arterial Disease

doi: 10.3390/biomedicines11071872

Figure Lengend Snippet: Characterization of BM-SC populations. ( A ) Representative dot plots show gating strategy of labeling live single-cell suspension analyzed by flow cytometry. ( B ) Representative histograms show that a single-cell suspension cultured for 14–18 days contained CD45 − CD90 + CD73 + CD105 + cells (MSCs), and dot plot shows CD45 + CD146 − cells (MACs and lymphocyte populations), CD45 − CD146 + cells (ECFCs) and other cell populations.

Article Snippet: BM-SCs were washed in phosphate-buffered saline and incubated for 30 min (4 °C) with the monoclonal antibodies allophycocyanin APC-labeled anti-CD146 (clone P1H12, BioLegend, San Diego, CA, USA), phycoerythrin PE-labeled anti-CD105 (clone SN6, Life Technologies, Carlsbad, CA, USA), fluorescein isothiocyanate FITC-labeled anti-CD45 (clone HI30, BioLegend), FITC-labeled anti-CD90 (clone 5E10, BioLegend), PE-labeled anti-CD73 (clone AD2, eBioscience, San Diego, CA, USA), and APC-labeled anti-CD45 (clone HI30, BioLegend).

Techniques: Labeling, Suspension, Flow Cytometry, Cell Culture

Journal: Research Square

Article Title: A Mutant Methionyl-tRNA Synthetase-Based toolkit to assess induced-Mesenchymal Stromal Cell secretome in mixed-culture disease models

doi: 10.21203/rs.3.rs-2838195/v1

Figure Lengend Snippet: Antibodies used in this manuscript.

Article Snippet: CD73 Monoclonal Antibody (AD2), APC , eBioscience (17-0739-41) , 1:20 (Flow cytometry (FC)).

Techniques: Immunocytochemistry, Western Blot, Flow Cytometry, Functional Assay, Antibody Labeling

Journal: iScience

Article Title: NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses

doi: 10.1016/j.isci.2021.102683

Figure Lengend Snippet:

Article Snippet: CD73 Monoclonal Antibody (AD2), eFluor 450, eBioscience™, Thermo Fisher Scientific , Thermo Fisher Scientific , Cat# 48-0739-42, RRID: AB_11041811.

Techniques: Produced, Recombinant, Lysis, Western Blot, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Derivative Assay, Software

Journal: International Journal of Molecular Sciences

Article Title: Changes in uPA, PAI-1, and TGF-β Production during Breast Cancer Cell Interaction with Human Mesenchymal Stroma/Stem-Like Cells (MSC)

doi: 10.3390/ijms20112630

Figure Lengend Snippet: Expression of typical mesenchymal stroma/stem-like cells (MSC) markers in MSC544. Isolated human MSC544 from a patient with a benign phyllodes tumor were long-term cultured (until P15 (for CD73) and P22 (for CD90 and CD105)), whereby the presence of surface markers that are associated with a mesenchymal stroma-/stem-like cell phenotype could be characterized.

Article Snippet: Following a PBS washing step, the cells were stained with a PE-labeled monoclonal mouse anti-human CD73 antibody (clone AD2) (BD Bioscience GmbH), a PE-labeled monoclonal CD90 antibody (clone 5E10) (BioLegend via Biozol GmbH, Eching, Germany), or a PE-labeled monoclonal mouse anti-human CD105 antibody (clone 43A3) (BioLegend via Biozol GmbH) at 4 °C for 15 min. A PE-labeled IgG1 antibody for CD73 or a double-labeled PE/FITC IgG1 antibody (both from Dako Denmark AS, Glostrup, Denmark) served as an appropriate control.

Techniques: Expressing, Isolation, Cell Culture